Zero padding will allow the use of either the standard 1K buffer or zero padded 2K, 4K, 8K or 16K data sets.
Increasing the zero padding has the effect of stretching the frequency axis which will allow more accurate measurement with the cursors.
The picture below shows the FFT of a 5V 3.472KHz square wave on channel A and a 4V 1KHz sine wave with no zero padding.
The picture below shows the same input signal with x4 zero padding. The scroll bar at the bottom of the screen may be used to scroll back and forth through the data buffer.